Fungal Genome Collection
University of Nebraska Lincoln
School of Biological Sciences and Center for Plant Science Innovation
Home About FGC Use Cases Species List


UniProt_SwissProt BLAST: Single locus
Species:
Saccharomyces cerevisiae S288C
Locus:
YMR216C
Length:
742
Number of sequences:
5887
Description:
YMR216C SKY1 SGDID:S000004829, Chr XIII from 701039-698811, Genome Release 64-1-1, reverse complement, Verified ORF, "SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1"
rec.SubjectHit LengthDescriptionAlign.LenE valueBit score% ident.  % pos.GO associations
421P39009    513   DUN1_YEAST DNA damage response protein kinase...1990.0003     47.8     25     39GO:0005634; C:nucleus; IDA:SGD.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
GO:0000077; P:DNA damage checkpoint; IMP:SGD.::GO:0048478; P:replication fork protection; IGI:SGD.
422D3ZSZ3    527   NLK_RAT Serine/threonine-protein kinase NLK O...1860.0003     47.8     28     45GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004707; F:MAP kinase activity; IEA:EC.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW.
423O54949    527   NLK_MOUSE Serine/threonine-protein kinase NLK...1860.0003     47.8     28     45GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0005634; C:nucleus; IDA:UniProtKB.
GO:0005524; F:ATP binding; IDA:UniProtKB.::GO:0000287; F:magnesium ion binding; IDA:UniProtKB.::GO:0004707; F:MAP kinase activity; IDA:UniProtKB.::GO:0042169; F:SH2 domain binding; IPI:UniProtKB.::GO:0008134; F:transcription factor binding; ISS:UniProtKB.::GO:0031625; F:ubiquitin protein ligase binding; ISS:UniProtKB.
GO:0030178; P:negative regulation of Wnt receptor signaling pathway; IMP:UniProtKB.::GO:0018107; P:peptidyl-threonine phosphorylation; IDA:UniProtKB.::GO:0046777; P:protein autophosphorylation; IDA:MGI.::GO:0006355; P:regulation of transcription, DNA-dependent; IMP:UniProtKB.::GO:0033136; P:serine phosphorylation of STAT3 protein; IDA:UniProtKB.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0007179; P:transforming growth factor beta receptor signaling pathway; ISS:UniProtKB.::GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW.
424Q9UBE8    527   NLK_HUMAN Serine/threonine-protein kinase NLK...1860.0003     47.8     28     45GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0005634; C:nucleus; ISS:UniProtKB.
GO:0005524; F:ATP binding; ISS:UniProtKB.::GO:0000287; F:magnesium ion binding; ISS:UniProtKB.::GO:0004707; F:MAP kinase activity; ISS:UniProtKB.::GO:0042169; F:SH2 domain binding; ISS:UniProtKB.::GO:0008134; F:transcription factor binding; IPI:UniProtKB.::GO:0031625; F:ubiquitin protein ligase binding; IPI:UniProtKB.
GO:0030178; P:negative regulation of Wnt receptor signaling pathway; ISS:UniProtKB.::GO:0018107; P:peptidyl-threonine phosphorylation; ISS:UniProtKB.::GO:0006355; P:regulation of transcription, DNA-dependent; ISS:UniProtKB.::GO:0033136; P:serine phosphorylation of STAT3 protein; ISS:UniProtKB.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0007179; P:transforming growth factor beta receptor signaling pathway; IMP:UniProtKB.::GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW.
425E1BMN8    534   NLK_BOVIN Serine/threonine-protein kinase NLK...1860.0003     47.8     28     45GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004707; F:MAP kinase activity; IEA:EC.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW.
426E2QWQ2    527   NLK_CANFA Serine/threonine-protein kinase NLK...1860.0003     47.8     28     45GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004707; F:MAP kinase activity; IEA:EC.::GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW.
427P38973    301   CC2H1_TRYBB Cell division control protein 2 h...1500.0003     47     29     53
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004693; F:cyclin-dependent protein kinase activity; IEA:EC.
428B0VXE8    423   CDK14_CALJA Cyclin-dependent kinase 14 OS=Cal...1910.0003     47.4     26     40GO:0000308; C:cytoplasmic cyclin-dependent protein kinase holoenzyme complex; ISS:UniProtKB.::GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.::GO:0005886; C:plasma membrane; ISS:UniProtKB.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004693; F:cyclin-dependent protein kinase activity; ISS:UniProtKB.
GO:0051301; P:cell division; IEA:UniProtKB-KW.::GO:0000086; P:G2/M transition of mitotic cell cycle; ISS:UniProtKB.::GO:0060828; P:regulation of canonical Wnt receptor signaling pathway; ISS:UniProtKB.::GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW.
429A4IIW7    423   CDK14_XENTR Cyclin-dependent kinase 14 OS=Xen...1900.0003     47.8     25     41GO:0000308; C:cytoplasmic cyclin-dependent protein kinase holoenzyme complex; ISS:UniProtKB.::GO:0005886; C:plasma membrane; ISS:UniProtKB.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004693; F:cyclin-dependent protein kinase activity; ISS:UniProtKB.
GO:0051301; P:cell division; IEA:UniProtKB-KW.::GO:0000086; P:G2/M transition of mitotic cell cycle; ISS:UniProtKB.::GO:0060828; P:regulation of canonical Wnt receptor signaling pathway; ISS:UniProtKB.::GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW.
430Q8W1D5    488   CIPKP_ARATH CBL-interacting serine/threonine-...1650.0003     47.8     28     48
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
GO:0050832; P:defense response to fungus; IEP:TAIR.::GO:0007165; P:signal transduction; IEA:InterPro.
431Q54JQ1    891   MKCC_DICDI Probable serine/threonine-protein ...1630.0003     48.1     25     37
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.::GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
432Q2QY53    438   CIPKW_ORYSJ CBL-interacting protein kinase 32...1600.0003     47.8     28     48
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
GO:0007165; P:signal transduction; IEA:InterPro.
433P74745    535   SPKC_SYNY3 Serine/threonine-protein kinase C ...1640.0003     47.8     29     49
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
434B4MXR8    787   PLK4_DROWI Serine/threonine-protein kinase PL...1190.0003     48.1     29     46GO:0005814; C:centriole; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.::GO:0004713; F:protein tyrosine kinase activity; IEA:InterPro.
435Q07014    512   LYN_RAT Tyrosine-protein kinase Lyn OS=Rattus...1740.0003     47.8     25     48GO:0034666; C:alpha2-beta1 integrin complex; IDA:RGD.::GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.::GO:0005624; C:membrane fraction; IDA:RGD.::GO:0045121; C:membrane raft; IDA:RGD.::GO:0030061; C:mitochondrial crista; IDA:RGD.::GO:0005758; C:mitochondrial intermembrane space; IDA:RGD.::GO:0005634; C:nucleus; ISS:UniProtKB.::GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.::GO:0014069; C:postsynaptic density; IDA:RGD.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0043208; F:glycosphingolipid binding; IPI:RGD.::GO:0005178; F:integrin binding; IDA:RGD.::GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:EC.::GO:0051219; F:phosphoprotein binding; IPI:RGD.::GO:0005161; F:platelet-derived growth factor receptor binding; IPI:RGD.::GO:0031625; F:ubiquitin protein ligase binding; IPI:RGD.
GO:0001782; P:B cell homeostasis; ISS:UniProtKB.::GO:0034605; P:cellular response to heat; IEP:RGD.::GO:0050663; P:cytokine secretion; IMP:RGD.::GO:0097028; P:dendritic cell differentiation; ISS:UniProtKB.::GO:0030218; P:erythrocyte differentiation; ISS:UniProtKB.::GO:0002774; P:Fc receptor mediated inhibitory signaling pathway; ISS:UniProtKB.::GO:0002431; P:Fc receptor mediated stimulatory signaling pathway; ISS:UniProtKB.::GO:0002553; P:histamine secretion by mast cell; IMP:RGD.::GO:0045087; P:innate immune response; IEA:UniProtKB-KW.::GO:0031663; P:lipopolysaccharide-mediated signaling pathway; ISS:UniProtKB.::GO:0070373; P:negative regulation of ERK1 and ERK2 cascade; ISS:UniProtKB.::GO:0043407; P:negative regulation of MAP kinase activity; ISS:UniProtKB.::GO:0070667; P:negative regulation of mast cell proliferation; ISS:UniProtKB.::GO:0001933; P:negative regulation of protein phosphorylation; ISS:UniProtKB.::GO:0034136; P:negative regulation of toll-like receptor 2 signaling pathway; ISS:UniProtKB.::GO:0034144; P:negative regulation of toll-like receptor 4 signaling pathway; ISS:UniProtKB.::GO:0014003; P:oligodendrocyte development; IEP:RGD.::GO:0002576; P:platelet degranulation; ISS:UniProtKB.::GO:2000670; P:positive regulation of dendritic cell apoptotic process; ISS:UniProtKB.::GO:0060369; P:positive regulation of Fc receptor mediated stimulatory signaling pathway; IMP:RGD.::GO:0060252; P:positive regulation of glial cell proliferation; IDA:RGD.::GO:0070447; P:positive regulation of oligodendrocyte progenitor proliferation; IMP:RGD.::GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; ISS:UniProtKB.::GO:0050855; P:regulation of B cell receptor signaling pathway; ISS:UniProtKB.::GO:0045646; P:regulation of erythrocyte differentiation; ISS:UniProtKB.::GO:0043304; P:regulation of mast cell degranulation; ISS:UniProtKB.::GO:0090330; P:regulation of platelet aggregation; ISS:UniProtKB.::GO:0051279; P:regulation of release of sequestered calcium ion into cytosol; IMP:RGD.::GO:0043200; P:response to amino acid stimulus; IEP:RGD.::GO:0048678; P:response to axon injury; IEP:RGD.::GO:0009743; P:response to carbohydrate stimulus; IEP:RGD.::GO:0042493; P:response to drug; IEP:RGD.::GO:0032868; P:response to insulin stimulus; IEP:RGD.::GO:0014070; P:response to organic cyclic compound; IEP:RGD.::GO:0006991; P:response to sterol depletion; IEP:RGD.::GO:0009636; P:response to toxin; IEP:RGD.::GO:0002513; P:tolerance induction to self antigen; ISS:UniProtKB.
436Q39023    370   MPK3_ARATH Mitogen-activated protein kinase 3...1750.0003     47.4     29     43GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004707; F:MAP kinase activity; IEA:EC.::GO:0005515; F:protein binding; IPI:UniProtKB.
GO:0009738; P:abscisic acid mediated signaling pathway; TAS:TAIR.::GO:0000169; P:activation of MAPK activity involved in osmosensory signaling pathway; IDA:TAIR.::GO:0010120; P:camalexin biosynthetic process; IMP:TAIR.::GO:0048481; P:ovule development; IGI:TAIR.::GO:0009626; P:plant-type hypersensitive response; IEA:UniProtKB-KW.::GO:0080136; P:priming of cellular response to stress; IMP:TAIR.::GO:2000038; P:regulation of stomatal complex development; IGI:TAIR.::GO:2000037; P:regulation of stomatal complex patterning; IGI:TAIR.::GO:0009617; P:response to bacterium; IEP:TAIR.::GO:0010200; P:response to chitin; IEP:TAIR.::GO:0009409; P:response to cold; IEP:TAIR.::GO:0006970; P:response to osmotic stress; IDA:TAIR.::GO:0006979; P:response to oxidative stress; IEP:TAIR.::GO:0010224; P:response to UV-B; IMP:TAIR.::GO:0009611; P:response to wounding; IEP:TAIR.
437Q6DE08    361   AUKBA_XENLA Aurora kinase B-A OS=Xenopus laev...1450.0003     47.4     26     50
438Q9FJV0    356   M2K6_ARATH Mitogen-activated protein kinase k...1420.0003     47.4     26     46
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004708; F:MAP kinase kinase activity; IDA:TAIR.::GO:0005515; F:protein binding; IPI:IntAct.::GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
439P32944    819   SWE1_YEAST Mitosis inhibitor protein kinase S...1540.0003     47.8     27     45GO:0005935; C:cellular bud neck; IDA:SGD.::GO:0005634; C:nucleus; IDA:SGD.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.::GO:0005515; F:protein binding; IPI:IntAct.::GO:0004674; F:protein serine/threonine kinase activity; IEA:InterPro.::GO:0004713; F:protein tyrosine kinase activity; IDA:SGD.
GO:0051301; P:cell division; IEA:UniProtKB-KW.::GO:0000078; P:cell shape checkpoint; IDA:SGD.::GO:0000086; P:G2/M transition of mitotic cell cycle; IMP:SGD.::GO:0007126; P:meiosis; IEA:UniProtKB-KW.::GO:0007067; P:mitosis; IEA:UniProtKB-KW.::GO:0010697; P:negative regulation of spindle pole body separation; IMP:SGD.::GO:0008361; P:regulation of cell size; IMP:SGD.::GO:0000079; P:regulation of cyclin-dependent protein kinase activity; IDA:SGD.::GO:0040020; P:regulation of meiosis; IGI:SGD.
440Q9M101    520   CDPKN_ARATH Calcium-dependent protein kinase ...1780.0003     47.8     24     38GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0005509; F:calcium ion binding; IEA:InterPro.::GO:0019903; F:protein phosphatase binding; IPI:UniProtKB.::GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
GO:0046686; P:response to cadmium ion; IEP:TAIR.
441O74135    439   CKI3_SCHPO Casein kinase I homolog 3 OS=Schiz...2000.0003     47.4     25     46GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004674; F:protein serine/threonine kinase activity; TAS:PomBase.
GO:0030100; P:regulation of endocytosis; ISS:PomBase.::GO:0007165; P:signal transduction; NAS:PomBase.
442Q9VPC0    952   KP58_DROME Serine/threonine-protein kinase PI...1670.0003     47.8     26     47GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004693; F:cyclin-dependent protein kinase activity; IEA:EC.
GO:0007155; P:cell adhesion; IMP:FlyBase.::GO:0008360; P:regulation of cell shape; IMP:FlyBase.
443P53599    1579   SSK2_YEAST MAP kinase kinase kinase SSK2 OS=S...1590.0003     48.1     31     49GO:0005935; C:cellular bud neck; IDA:SGD.::GO:0005934; C:cellular bud tip; IDA:SGD.::GO:0005829; C:cytosol; IDA:SGD.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004709; F:MAP kinase kinase kinase activity; IDA:SGD.::GO:0005515; F:protein binding; IPI:IntAct.
GO:0030036; P:actin cytoskeleton organization; IDA:SGD.::GO:0000161; P:MAPK cascade involved in osmosensory signaling pathway; IMP:SGD.::GO:0007234; P:osmosensory signaling pathway via two-component system; IPI:SGD.::GO:0046777; P:protein autophosphorylation; IDA:SGD.
444Q1KTF2    357   HOG1_MYCGM Mitogen-activated protein kinase H...1500.0004     47     27     47GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004707; F:MAP kinase activity; IEA:EC.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
445Q1DUU8    364   HOG1_COCIM Mitogen-activated protein kinase H...1490.0004     47     27     47GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004707; F:MAP kinase activity; IEA:EC.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
446Q875L0    358   HOG1_CRYPA Mitogen-activated protein kinase H...1500.0004     47     27     48GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004707; F:MAP kinase activity; IEA:EC.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
447Q96TL5    358   HOG1_NEUCR Mitogen-activated protein kinase h...1500.0004     47     27     48GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004707; F:MAP kinase activity; IEA:EC.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
448Q0CIC7    370   MPKC_ASPTN Mitogen-activated protein kinase m...1510.0004     47     29     47
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004707; F:MAP kinase activity; IEA:EC.
449Q5JK68    513   CDKC2_ORYSJ Cyclin-dependent kinase C-2 OS=Or...1690.0004     47.4     27     48
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004693; F:cyclin-dependent protein kinase activity; IEA:EC.::GO:0008353; F:RNA polymerase II carboxy-terminal domain kinase activity; IEA:EC.
450A8WIP6    344   CDK20_DANRE Cyclin-dependent kinase 20 OS=Dan...1490.0004     47     30     48GO:0005929; C:cilium; IEA:UniProtKB-SubCell.::GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004693; F:cyclin-dependent protein kinase activity; IEA:EC.
GO:0051301; P:cell division; IEA:UniProtKB-KW.::GO:0060271; P:cilium morphogenesis; IMP:ZFIN.::GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
451B0VXL7    451   CDK14_CALMO Cyclin-dependent kinase 14 OS=Cal...1910.0004     47.4     26     40GO:0000308; C:cytoplasmic cyclin-dependent protein kinase holoenzyme complex; ISS:UniProtKB.::GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.::GO:0005886; C:plasma membrane; ISS:UniProtKB.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.::GO:0004693; F:cyclin-dependent protein kinase activity; ISS:UniProtKB.
GO:0051301; P:cell division; IEA:UniProtKB-KW.::GO:0000086; P:G2/M transition of mitotic cell cycle; ISS:UniProtKB.::GO:0060828; P:regulation of canonical Wnt receptor signaling pathway; ISS:UniProtKB.::GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW.
records
Previous ‹‹ ›› Next Total records: 1000 421 - 450
Elimate unknown annotation:
Filter for keyword on hit description:
Select upper E value:
Select lower bit score:
Select lower %idenity value:
Select lower %positive value:
Taxonomic division:
Lower limit on hit length:
Lower limit on alignment length::