Fungal Genome Collection
University of Nebraska Lincoln
School of Biological Sciences and Center for Plant Science Innovation
Home About FGC Use Cases Species List


UniProt_SwissProt BLAST: Single locus
Species:
Saccharomyces cerevisiae S288C
Locus:
YKL062W
Length:
630
Number of sequences:
5887
Description:
YKL062W MSN4 SGDID:S000001545, Chr XI from 323228-325120, Genome Release 64-1-1, Verified ORF, "Transcriptional activator related to Msn2p; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression"
rec.SubjectHit LengthDescriptionAlign.LenE valueBit score% ident.  % pos.GO associations
31P08154    508   EGR1_RAT Early growth response protein 1 OS=R...620.0000000004     66.2     42     66GO:0005737; C:cytoplasm; IDA:UniProtKB.::GO:0005634; C:nucleus; IDA:UniProtKB.
GO:0003690; F:double-stranded DNA binding; IDA:RGD.::GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IDA:RGD.::GO:0000979; F:RNA polymerase II core promoter sequence-specific DNA binding; IDA:RGD.::GO:0008134; F:transcription factor binding; IPI:RGD.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0071236; P:cellular response to antibiotic; IEP:RGD.::GO:0071320; P:cellular response to cAMP; IEP:RGD.::GO:0035690; P:cellular response to drug; IEP:RGD.::GO:0071372; P:cellular response to follicle-stimulating hormone stimulus; IEP:RGD.::GO:0071363; P:cellular response to growth factor stimulus; IEP:RGD.::GO:0071504; P:cellular response to heparin; IDA:UniProtKB.::GO:0071455; P:cellular response to hyperoxia; IEP:RGD.::GO:0071456; P:cellular response to hypoxia; IEP:RGD.::GO:0032869; P:cellular response to insulin stimulus; IEP:RGD.::GO:0071317; P:cellular response to isoquinoline alkaloid; IEP:RGD.::GO:0071260; P:cellular response to mechanical stimulus; IEP:RGD.::GO:0071506; P:cellular response to mycophenolic acid; IDA:UniProtKB.::GO:0071383; P:cellular response to steroid hormone stimulus; IEP:RGD.::GO:0007623; P:circadian rhythm; IEP:RGD.::GO:0072110; P:glomerular mesangial cell proliferation; IMP:UniProtKB.::GO:0007611; P:learning or memory; IMP:RGD.::GO:0060291; P:long-term synaptic potentiation; IEP:RGD.::GO:0048709; P:oligodendrocyte differentiation; IEP:RGD.::GO:0072303; P:positive regulation of glomerular metanephric mesangial cell proliferation; IMP:UniProtKB.::GO:0043525; P:positive regulation of neuron apoptotic process; IMP:RGD.::GO:0048169; P:regulation of long-term neuronal synaptic plasticity; IMP:RGD.::GO:0001975; P:response to amphetamine; IEP:RGD.::GO:0034465; P:response to carbon monoxide; IEP:RGD.::GO:0042220; P:response to cocaine; IEP:RGD.::GO:0051602; P:response to electrical stimulus; IEP:RGD.::GO:0045471; P:response to ethanol; IEP:RGD.::GO:0071873; P:response to norepinephrine stimulus; IEP:RGD.::GO:0031667; P:response to nutrient levels; IEP:RGD.
32B0XSK6    424   CREA_ASPFC Probable DNA-binding protein creA ...590.00000000001     70.9     51     69GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
33A2QCJ9    427   CREA_ASPNC Probable DNA-binding protein creA ...590.00000000001     70.9     51     69GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
34A1C6L9    425   CREA_ASPCL Probable DNA-binding protein creA ...590.00000000001     70.9     51     69GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
35Q4X0Z3    424   CREA_ASPFU Probable DNA-binding protein creA ...590.00000000001     70.9     51     69GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
36A1DH89    416   CREA_NEOFI Probable DNA-binding protein creA ...590.00000000001     70.5     51     69GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
37Q05620    427   CREA_ASPNG DNA-binding protein creA OS=Asperg...590.00000000001     70.9     51     69GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
38O73692    194   EGR1_COTJA Early growth response protein 1 OS...530.00000001     58.9     45     68GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0043565; F:sequence-specific DNA binding; ISS:AgBase.::GO:0003700; F:sequence-specific DNA binding transcription factor activity; ISS:AgBase.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0045893; P:positive regulation of transcription, DNA-dependent; ISS:AgBase.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
39Q01793    74   KRUP_TRICA Protein krueppel OS=Tribolium cast...290.047     37.7     41     66GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0035282; P:segmentation; IEA:UniProtKB-KW.
40Q05159    412   EGR2B_DANRE Early growth response protein 2b ...850.000000003     62.8     38     58GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
41A1XSY8    471   EGR2_PIG E3 SUMO-protein ligase EGR2 OS=Sus s...850.000000001     64.3     38     58GO:0005737; C:cytoplasm; ISS:UniProtKB.::GO:0005634; C:nucleus; ISS:UniProtKB.
GO:0003682; F:chromatin binding; ISS:UniProtKB.::GO:0016874; F:ligase activity; IEA:UniProtKB-KW.::GO:0003700; F:sequence-specific DNA binding transcription factor activity; ISS:UniProtKB.::GO:0044212; F:transcription regulatory region DNA binding; ISS:UniProtKB.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006611; P:protein export from nucleus; ISS:UniProtKB.
42P08152    470   EGR2_MOUSE E3 SUMO-protein ligase EGR2 OS=Mus...1080.0000000005     65.9     36     56GO:0005737; C:cytoplasm; IDA:UniProtKB.::GO:0005634; C:nucleus; IDA:UniProtKB.
GO:0003682; F:chromatin binding; IDA:UniProtKB.::GO:0016874; F:ligase activity; IEA:UniProtKB-KW.::GO:0005515; F:protein binding; IPI:MGI.::GO:0001102; F:RNA polymerase II activating transcription factor binding; IPI:BHF-UCL.::GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.::GO:0044212; F:transcription regulatory region DNA binding; IDA:UniProtKB.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0035284; P:brain segmentation; IMP:MGI.::GO:0071310; P:cellular response to organic substance; IDA:MGI.::GO:0021612; P:facial nerve structural organization; IGI:MGI.::GO:0045444; P:fat cell differentiation; IMP:BHF-UCL.::GO:0008045; P:motor axon guidance; IGI:MGI.::GO:0042552; P:myelination; IMP:MGI.::GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:MGI.::GO:0006611; P:protein export from nucleus; IDA:UniProtKB.::GO:0030278; P:regulation of ossification; IMP:MGI.::GO:0021660; P:rhombomere 3 formation; IMP:MGI.::GO:0021666; P:rhombomere 5 formation; IMP:MGI.::GO:0007622; P:rhythmic behavior; IDA:MGI.::GO:0014037; P:Schwann cell differentiation; IMP:MGI.::GO:0006366; P:transcription from RNA polymerase II promoter; IDA:BHF-UCL.
43P51774    470   EGR2_RAT E3 SUMO-protein ligase EGR2 OS=Rattu...1080.0000000005     65.5     36     56GO:0005737; C:cytoplasm; ISS:UniProtKB.::GO:0005634; C:nucleus; ISS:UniProtKB.
GO:0003682; F:chromatin binding; ISS:UniProtKB.::GO:0071837; F:HMG box domain binding; IPI:UniProtKB.::GO:0016874; F:ligase activity; IEA:UniProtKB-KW.::GO:0044212; F:transcription regulatory region DNA binding; ISS:UniProtKB.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0007611; P:learning or memory; IEP:RGD.::GO:0006611; P:protein export from nucleus; ISS:UniProtKB.::GO:0048168; P:regulation of neuronal synaptic plasticity; IEP:RGD.::GO:0032868; P:response to insulin stimulus; IMP:RGD.
44O73691    194   EGR1_CHICK Early growth response protein 1 OS...470.00000002     58.2     49     74GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0043565; F:sequence-specific DNA binding; ISS:AgBase.::GO:0008270; F:zinc ion binding; IEA:InterPro.
45P11161    476   EGR2_HUMAN E3 SUMO-protein ligase EGR2 OS=Hom...850.000000002     63.5     38     58GO:0005737; C:cytoplasm; ISS:UniProtKB.::GO:0005634; C:nucleus; ISS:UniProtKB.
GO:0003682; F:chromatin binding; ISS:UniProtKB.::GO:0016874; F:ligase activity; IEA:UniProtKB-KW.::GO:0001102; F:RNA polymerase II activating transcription factor binding; ISS:BHF-UCL.::GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.::GO:0044212; F:transcription regulatory region DNA binding; ISS:UniProtKB.::GO:0031625; F:ubiquitin protein ligase binding; IPI:UniProtKB.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0045444; P:fat cell differentiation; ISS:BHF-UCL.::GO:0006611; P:protein export from nucleus; ISS:UniProtKB.::GO:0006366; P:transcription from RNA polymerase II promoter; ISS:BHF-UCL.
46Q24266    644   BTD_DROME Transcription factor btd OS=Drosoph...480.00000002     60.8     50     71GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IDA:FlyBase.::GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.::GO:0008134; F:transcription factor binding; IPI:FlyBase.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0035288; P:anterior head segmentation; TAS:FlyBase.::GO:0007376; P:cephalic furrow formation; IMP:FlyBase.::GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:FlyBase.::GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:FlyBase.::GO:0035289; P:posterior head segmentation; TAS:FlyBase.
47Q08427    421   EGR2_XENLA Early growth response protein 2 OS...850.000000005     62.4     38     58GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
48Q01792    74   KRUP_SCICO Protein krueppel OS=Sciara coproph...290.072     37     41     69GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0035282; P:segmentation; IEA:UniProtKB-KW.
49P50898    474   MIG1_KLULA Regulatory protein MIG1 OS=1267 / ...640.00000000007     68.6     48     69GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.::GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
50B5DE03    413   WT1_XENTR Wilms tumor protein homolog OS=Xeno...560.0000000004     65.9     54     71GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0003723; F:RNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0072013; P:glomus development; ISS:UniProtKB.::GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.::GO:0030178; P:negative regulation of Wnt receptor signaling pathway; ISS:UniProtKB.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW.
51P79958    407   WT1B_XENLA Wilms tumor protein homolog B OS=X...560.0000000004     65.9     54     71GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0003723; F:RNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0072013; P:glomus development; IMP:UniProtKB.::GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.::GO:0030178; P:negative regulation of Wnt receptor signaling pathway; ISS:UniProtKB.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW.
52Q4R8H9    653   ZN274_MACFA Neurotrophin receptor-interacting...550.0009     46.2     33     60GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0016032; P:viral reproduction; IEA:InterPro.
53Q96GC6    653   ZN274_HUMAN Neurotrophin receptor-interacting...550.0004     47     33     62GO:0005813; C:centrosome; IDA:HPA.::GO:0005730; C:nucleolus; TAS:ProtInc.
GO:0005515; F:protein binding; IPI:UniProtKB.::GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.::GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.::GO:0003714; F:transcription corepressor activity; TAS:ProtInc.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0016032; P:viral reproduction; IEA:InterPro.
54A6NM28    416   ZFP92_HUMAN Zinc finger protein 92 homolog OS...700.0002     48.1     34     53GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
55Q02446    784   SP4_HUMAN Transcription factor Sp4 OS=Homo sa...670.0000000009     65.5     46     61GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0003713; F:transcription coactivator activity; TAS:ProtInc.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
56P08047    785   SP1_HUMAN Transcription factor Sp1 OS=Homo sa...720.00000001     61.6     39     56GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0005634; C:nucleus; IC:UniProtKB.
GO:0043425; F:bHLH transcription factor binding; ISS:BHF-UCL.::GO:0003690; F:double-stranded DNA binding; IDA:BHF-UCL.::GO:0042826; F:histone deacetylase binding; IPI:BHF-UCL.::GO:0071837; F:HMG box domain binding; IPI:UniProtKB.::GO:0008022; F:protein C-terminus binding; IPI:UniProtKB.::GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.::GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; ISS:BHF-UCL.::GO:0001103; F:RNA polymerase II repressing transcription factor binding; ISS:BHF-UCL.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0043923; P:positive regulation by host of viral transcription; IDA:UniProtKB.
57P46974    1380   RSF2_YEAST Respiration factor 2 OS=Saccharomy...740.0000000001     68.6     42     61GO:0005634; C:nucleus; IDA:SGD.
GO:0043565; F:sequence-specific DNA binding; IDA:SGD.::GO:0003700; F:sequence-specific DNA binding transcription factor activity; ISS:SGD.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
58Q90WR8    771   SP3_CHICK Transcription factor Sp3 OS=Gallus ...610.0000000009     65.5     44     64GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.::GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
59O89090    784   SP1_MOUSE Transcription factor Sp1 OS=Mus mus...720.00000002     61.2     39     56GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0005634; C:nucleus; IDA:MGI.
GO:0043425; F:bHLH transcription factor binding; IPI:BHF-UCL.::GO:0003690; F:double-stranded DNA binding; IDA:MGI.::GO:0035326; F:enhancer binding; IDA:MGI.::GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IDA:BHF-UCL.::GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IDA:MGI.::GO:0000979; F:RNA polymerase II core promoter sequence-specific DNA binding; IDA:MGI.::GO:0001103; F:RNA polymerase II repressing transcription factor binding; IPI:BHF-UCL.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0060216; P:definitive hemopoiesis; IGI:MGI.::GO:0048596; P:embryonic camera-type eye morphogenesis; IGI:MGI.::GO:0001892; P:embryonic placenta development; IGI:MGI.::GO:0060136; P:embryonic process involved in female pregnancy; IGI:MGI.::GO:0048706; P:embryonic skeletal system development; IGI:MGI.::GO:0043353; P:enucleate erythrocyte differentiation; IGI:MGI.::GO:0001889; P:liver development; IGI:MGI.::GO:0030324; P:lung development; IGI:MGI.::GO:0030219; P:megakaryocyte differentiation; IGI:MGI.::GO:0001503; P:ossification; IGI:MGI.::GO:0001829; P:trophectodermal cell differentiation; IGI:MGI.
60Q01714    786   SP1_RAT Transcription factor Sp1 OS=Rattus no...670.00000002     61.2     39     57GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.::GO:0005634; C:nucleus; IDA:RGD.::GO:0032993; C:protein-DNA complex; IDA:RGD.
GO:0003690; F:double-stranded DNA binding; IDA:RGD.::GO:0035035; F:histone acetyltransferase binding; IPI:RGD.::GO:0008270; F:zinc ion binding; IEA:InterPro.
61P31507    74   KRUP_APIME Protein krueppel OS=Apis mellifera...290.11     36.6     41     66GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.::GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0035282; P:segmentation; IEA:UniProtKB-KW.
records
Previous ‹‹ ›› Next Total records: 1000 31 - 60
Elimate unknown annotation:
Filter for keyword on hit description:
Select upper E value:
Select lower bit score:
Select lower %idenity value:
Select lower %positive value:
Taxonomic division:
Lower limit on hit length:
Lower limit on alignment length::