— Supplementary data —

[Supplementary tables and figures included with the manuscript]

• Table S1: Organisms found to have sequences similar to Osiris proteins
• Table S2 (Excel file): List of all Osiris genes identified from the 13 complete insect genomes
• Table S3: List of 24 Osiris genes in the D. melanogaster genome and those identified from the 11 other Drosophila genomes
• Table S4: List of data sources for the arthropod genomes used in this study
• Figure S1: Alignment of D. melanogaster Osiris protein sequences
• Figure S2: Maximum likelihood phylogeny of Osiris proteins reonstructed by FastTree

[Osiris protein sequences in FASTA format]

See Supplementary Table S2 for the description of each sequence

• All Osiris protein sequences used in the study
  - Osi10 sequences included are divided into 10a and 10b for better alignments.
  Osi_all.fa
• Osi 10 protein sequences as annotated (without dividing into two parts)
  Osi_10.fa
• Osiris protein sequences translated from the contigs assembled from Lepismachilis y-signata ESTs
  Osi_Lepis.fa

[Osiris protein alignments]

All alignments were generated using Mafft with L-INS-i algorithm

• Alignment including all sequences
  - This alignment was used to reconstruct the phylogeny in Supplementary Figure S2
  Osi_all_Lep.mafft (FASTA format)
  Osi_all_Lep.phy (Phylip format)
• Alignment including only D. melanogaster Osiris sequences
  - D. melanogaster sequences were extracted from the above alignment
  - This alignment was used to generate Supplementary Figure S1
  Osi_dmel.aln (Clustal format)
  Osi_dmel.mafft (FASTA format)
  Osi_dmel.phy (Phylip format)
  

[MEME motif analysis for Drosophila melanogaster Osiris proteins]

MEME analysis was done using http://meme.sdsc.edu/meme/cgi-bin/meme.cgi

• D. melanogaster Osiris proteins in FASTA format
  Osi_dmel.fa
• MEME analysis result for D. melanogaster Osiris proteins
  - MEME training and motif discovery was performed using Osi1-Osi23
  Osi_dmel_MEME.webarchive (downlaodable for Macintosh browser)
  Osi_dmel_MEME.html (click this link for other browser)
  
• MAST analysis result for D. melanogaster Osiris proteins (including Osi24)
  Osi_dmel_MAST.webarchive (downloadable for Macintosh browser)
  Osi_dmel_MAST.html (click this link for other browser)
  

NOTE1: Correspondence of MEME motifs and the Osiris signatures is as follows.
- 2-Cys domain: Motif10 (not always) + Motif5
- DUF1676: Motif3 + Motif2 + Motif8 + Motif1
- AQXLAY motif: Motif4

NOTE2: The above MEME result webarchive file (or html link) can be used to search the ten Osiris motifs in your own sequences. Download the webarchive and open it in any browser (or click the html link in any browser), then click on the "MAST" button at the bottom of the "Discovered Motifs" section to submit your sequences.

— References —

• Dorer, D.R., Rudnick, J. A., Moriyama, E. N. and Christensen, A. C. (2003) A family of genes clustered at the Triplo-lethal locus of Drosophila melanogaster has an unusual evolutionary history and significant synteny with Anopheles gambiae. Genetics, 165: 613-621.
• Dorer, D. R., Cadden, M. A., Gordesky-Gold, B., Harries, G. and Christensen, A. C. (1993) Suppression of a lethal trisomic phenotype in Drosophila melanogaster by increased dosage of an unlinked locus. Genetics, 134: 243-249.
• Read about "Myth of Osiris and Isis" on Wikipedia